FOXG1 Directly Suppresses Wnt5a During the Development of the Hippocampus / 神经科学通报·英文版

The Wnt signaling pathway plays key roles in various developmental processes. Wnt5a, which activates the non-canonical pathway, has been shown to be particularly important for axon guidance and outgrowth as well as dendrite morphogenesis. However, the mechanism underlying the regulation of Wnt5a remains unclear. Here, through conditional disruption of Foxg1 in hippocampal progenitors and postmitotic neurons achieved by crossing Foxg1

Optimization of a fluorescent qPCR detection for RNA of SARS-CoV-2 / 生物工程学报

We optimized a fluorescent quantitative polymerase chain reaction (qPCR) assay system for rapid and real time detection of SARS-CoV-2 RNA. The results show that the lowest dilution of RNA samples used for the detection of SARS-CoV-2 RNA could reach 1/10 000 (the initial value is set as 10 ng/μL). Moreover, the cycle threshold (Ct) for samples of clinically diagnosed COVID-19 was lower than 35 or 40. The sensitivity of this method was satisfactory. The results were consistent with those of the COVID-19 detection kit on the market under the same conditions, but the number of cycles required was shortened by about 2. Therefore, the optimized assay developed in this study can be used in screening and early clinical diagnosis. Our work provides a tool to facilitate rapid clinical diagnosis of COVID-19.

Electroacupuncture Alleviates Motor Symptoms and Up-Regulates Vesicular Glutamatergic Transporter 1 Expression in the Subthalamic Nucleus in a Unilateral 6-Hydroxydopamine-Lesioned Hemi-Parkinsonian Rat Model / 神经科学通报·英文版

Previous studies have shown that electroacupuncture (EA) promotes recovery of motor function in Parkinson's disease (PD). However the mechanisms are not completely understood. Clinically, the subthalamic nucleus (STN) is a critical target for deep brain stimulation treatment of PD, and vesicular glutamate transporter 1 (VGluT1) plays an important role in the modulation of glutamate in the STN derived from the cortex. In this study, a 6-hydroxydopamine (6-OHDA)-lesioned rat model of PD was treated with 100 Hz EA for 4 weeks. Immunohistochemical analysis of tyrosine hydroxylase (TH) showed that EA treatment had no effect on TH expression in the ipsilateral striatum or substantia nigra pars compacta, though it alleviated several of the parkinsonian motor symptoms. Compared with the hemi-parkinsonian rats without EA treatment, the 100 Hz EA treatment significantly decreased apomorphine-induced rotation and increased the latency in the Rotarod test. Notably, the EA treatment reversed the 6-OHDA-induced down-regulation of VGluT1 in the STN. The results demonstrated that EA alleviated motor symptoms and up-regulated VGluT1 in the ipsilateral STN of hemi-parkinsonian rats, suggesting that up-regulation of VGluT1 in the STN may be related to the effects of EA on parkinsonian motor symptoms via restoration of function in the cortico-STN pathway.

Relationship Between Maximum Standardized Uptake Value of 18F-FDG PET/CT and Clinical Features of Tongue Squamous Cell Carcinoma / 中国医学影像学杂志

Purpose To investigate the relationship between maximum standardized uptake value (SUVmax) of 18F-FDG PET/CT and clinical features of tongue squamous cell carcinoma (TSCC),in order to provide better PET/CT results for clinical guide.Materials and Methods Fifty-two patients with pathologically confirmed TSCC who accepting PET/CT examination before surgery were retrospectively analyzed.Single-factor analysis and multiple regression analysis were conducted on possible factors influencing primary tumor SUVmax,including gender,age,smoking history,tumor location,tumor size,TNM stage,T stage and N stage.Results Single-analysis showed that SUVmax was correlated with gender,tumor location,tumor size,TNM stage,T stage and N stage (P＜0.05),and was not correlated with tumor differentiation,smoking history and age (P＞0.05).Multivariate linear regression analysis showed that gender,tumor location,tumor size,T stage and N stage were independent influencing factors of primary tumor SUVmax (P＜0.05).Primary tumor SUVmax had predictive value for lymph node metastasis.When the cutoff value was 6.57,the diagnostic efficiency was the highest,the sensitivity was 79.2％ and the specificity was 85.7％.Conclusion TSCC 18F-FDG PET/CT SUVmax is higher among male patients with tongue base tumor location,larger tumor size and lymph node metastasis.Primary tumor SUVmax is of important significance in predicting lymph node metastasis.

CT anatomic study of the persistent foramen Huschke and the weakness of the remains of the foramen Huschke / 实用放射学杂志

Objective To study the morphology of the persistent foramen of Huschke (PHF)and the weakness of the remains of foramen of Huschke (WRHF)in the external auditory canal (EAC)by high resolution computer tomography (HRCT)in vivo. Methods The thin HRCT images of 525 cases elder than 5 years were retrospectively reviewed by two radiologists,to detect the PHF or WRHF,and to depict the precise location,size and the distance to the tympanic membrane.The incidence,sex differences, and morphologic data of PHF and WRHF were statistical analyzed.Results ①PHFs were found in 146 EACs of 40 males and 77 fe-males with the prevalence of 13.91%,and were found on both sides in 29 cases.Mean maximum axial diameter was 2.35 mm± 1.1 6 mm (0.6-5.8 mm)and sagittal diameter was 2.09 mm±1.2 mm (0.5-6.2 mm).Mean distance between the inner edge of PFH and the sulcus tympanicus was 1.61 mm±2.4 mm (0-10 mm).②WRHFs were found in 233 EACs of 58 males and 122 fe-males with the prevalence of 22.1 9%,and were found on both sides in 53 cases.Mean maximum axial diameter was 2.43 mm± 0.89 mm (0.8-5.4 mm)and sagittal diameter was 2.08 mm±0.73 mm (0.6-4.3 mm).Mean distance between the inner edge of WRPFH and the sulcus tympanicus was 1.55 mm±1.2 mm (0 - 9.9 mm).③The distance to the sulcus tympanicus was within 3 mm in 137 cases (90.73%)of PFHs and 221 cases (94.43%)of WRFHs.Conclusion Both PHF and WRFH are common by the CT morphological study in vivo.The medial part of EAC is predominant and female is predominant.The incidence and the location of PHF and WRFH are no significant difference.

Neural stem cell transplantation for cerebral palsy: nerve repair and safety evaluation / 中国组织工程研究

BACKGROUND:Neural stem cels can repair the damaged brain tissues with potentials of proliferation and differentiation, which become one of the important directions for treating cerebral palsy. OBJECTIVE:To observe the clinical effect and safety of neural stem cel transplantation on the treatment of cerebral palsy in children. METHODS:Neural stem cels were isolated from human embryonic brain and identified by immunofluorescence staining, which were transplanted intravenously into 26 children with cerebral palsy. Children's motor functions were evaluated by gross motor function measure scale and Peabody development motor scale-fine motor scale before treatment, and 3 and 6 months after treatment. Routine blood test and liver-kidney function were detected before and after treatment. Clinical adverse reactions in children with cerebral palsy were monitored. RESULTS AND CONCLUSION:The lost cases were not found during 6 months of folow-up. Specific proteins of neural stem cels were al positive in this study. At 3 and 6 months after transplantation, the A, B, C functional area scores and total score on the gross motor function measure scale were obviously increased (P 0.05). At 3 months after transplantation, the fine motor quotient, grasping subtest and visual-motor integration were not remarkably increased (P > 0.05); these scores, however, were elevated after 6 months with statistical significance (P < 0.05, P < 0.01). The results of routine blood test and liver-kidney function in 26 children were in normal range, and there were no serious adverse reactions during the cel transplantation. Therefore, neural stem cel transplantation has high safety and good curative effects to improve the motor function of children with severe cerebral palsy, especialy for gross motors.

Relationship of Th17 cells as well as interleukin (IL)-17A and IL-23R mRNA with psoriasis vulgaris / 中华皮肤科杂志

Objective To assess the number of peripheral blood Th17 cells and mRNA expressions of IL-17A and IL-23R and their correlations with disease severity in patients with psoriasis vulgaris (PV).Methods Tissue specimens were resected from the lesions of 25 patients with PV and normal skin of 10 human controls,and venous blood samples were obtained from 20 of the patients and all of the normal human controls.Reverse transcription PCR and flow cytometry were carried out to measure the mRNA levels of IL-17A and IL-23R in these tissue specimens and quantify the number of peripheral blood CD4+IL-17+ T lymphocytes.Psoriasis area and severity index (PASI) was calculated for these patients.Results A significant increase was observed in the IL-17A and IL-23R mRNA levels in the patients compared with the controls (0.996 ± 0.231vs.0.437 ± 0.096,t =10.572,P ＜ 0.05; 1.006 ± 0.339 vs.0.491 ± 0.196,t =6.015,P ＜ 0.05).The levels of both IL-17A mRNA and IL-23R mRNA were positively correlated with PASI (r,=0.67,0.70,respectively,both P ＜ 0.05).The number of CD4+IL-1 7+ T lymphocytes in peripheral blood showed no significant differences between the patients and controls.No statistical correlation was observed between the counts of CD4+IL-17+ T lymphocytes and expression levels of IL-17A or IL-23R mRNA in psoriatic lesions.Conclusions In patients with PV,there is an increase in the expressions of IL-17A and IL-23R mRNA in lesions,which are correlated with disease severity,while no significant change is observed in the number of peripheral blood CD4+IL-17+ T cells.

Chlamydia trachomatis heat shock protein 60 (cHSP60) induces murine cervicitis / 中华皮肤科杂志

Objective To investigate the role of cHSP60 in the pathogenesis of murine cervicitis.Methods Fifty female C3H/HeN mice were randomly and equally classified into 5 groups, including the control group receiving no treatment and 4 groups receiving intravaginal inoculation of cHSP60 (cHSP60 group),live elementary bodies of Chlamydia trachomatis mouse pneumonitis (MoPn group), inactive elementary bodies of MoPn (inactive MoPn group) and growth medium (medium group), respectively. Five days after the inoculation,cervical tissue was resected from these mice and subjected to pathological examination. Results There were varying degrees of inflammatory reaction characterized by neutrophil infiltration, necrosis and shedding of mucosal cells in the cervices of mice in cGSP60 and MoPn groups. No statistical difference was observed in the incidence of cervicitis (90% vs. 80%, P ＞ 0.05), neutrophile count [76.00 (25.0 - 80.0) vs. 25.00 (8.75 -32.5), P＞ 0.05] or inflammation score [12.5 (11.5 - 14.25) vs. 9.00 (8.00 - 11.5), P ＞ 0.05] between the cHSP60 and MoPn group. The inflammatory reaction was weak with decreased incidence of cervicitis (40%),inflammation score [0.00 (0.00- 12.50)] and neutrophile count [0.00 (0.00- 15.50)] in inactive MoPn group compared with the cHSP60 and MoPn groups (all P ＜ 0.05). A small number of neutrophils migrated into the superficial layer of cervical mucosa in only 2 mice in the medium group. Conclusion cHSP60 may be a primary pathogenic factor in chlamydial genital tract infection.

Protsome analysis of the total protein in Vibrio cholerae by 2-DE / 中华微生物学和免疫学杂志

Objective To study the different proteins of human and environment Vibrio cholerae (V. cholerae) by two dimensional electrophoresis (2-DE) and TOF-TOF-MS. Methods Total cellular pro-tein was extracted by lysate from V. cholerae, and proteins were separated by 2-DE under immobilized pH gradients(IPG), then electrophoregrams were dealed with coomassie brilliant blue, and analyzed by Im-ageMaster 2D Elite 5.0, finally the different protein spots were identified by TOF-TOF-MS. Results High repetitive 2-DE maps were obtained. 2-DE and image analysis revealed 1032±22 protein spots, and PI value was among 4.00 to 7.20. Matching spots were 1025±24 in two repeats electrophoregrams and matching ratio was 96.30%. Conclusion The different protein spots were successfully established with high quality and sharpness separation by 2-DE and TOF-TOF-MS, which stands as a valuable resource for proteomics re-search of V. cholerae.

Nonlinear inverse modeling of sensor based on back-propagation fuzzy logical system / 药物分析学报

Objective To correct the nonlinear error of sensor output, a new approach to sensor inverse modeling based on Back-Propagation Fuzzy Logical System (BP FS) is presented. Methods The BP FS is a computationally efficient nonlinear universal approximator, which is capable of implementing complex nonlinear mapping from its input pattern space to the output with fast convergence speed. Results The neuro-fuzzy hybrid system, i.e. BP FS, is then applied to construct nonlinear inverse model of pressure sensor. The experimental results show that the proposed inverse modeling method automatically compensates the associated nonlinear error in pressure estimation, and thus the performance of pressure sensor is significantly improved. Conclusion The proposed method can be widely used in nonlinearity correction of various kinds of sensors to compensate the effects of nonlinearity and temperature on sensor output.

Recognizing frequency characteristics of gas sensor array / 药物分析学报

A novel method based on independent component analyzing (ICA) in frequency domain to distinguish the frequency characteristics of multi-sensor system is presented. The conditions of this type of ICA are considered and each step of resolving the problem is discussed. For a two gas sensor array, the frequency characteristics including amplitude-frequency and phase-frequency are recognized by this method, and cross-sensitivity between them is also eliminated. From the principle of similarity, the recognition mean square error is no more than 0.085.

The exploration on teaching method of clinical neuroanatomy / 中华医学教育探索杂志

Clinical neuroanatomy is a bridge between basic medicine and clinic. To resolve the deficiency existing in PBL teaching,we improved the basic teaching installation and teaching practice and evaluation system. The results indicated that we had gotten satisfactory effect by using teaching method of clinical neuroanatomy

EXPRESSION OF GFAP IN RAT HYPOTHALAMUS AFTER DIFFERENT TEMPERATURE HEAT STRESSES / 解剖学报

Objective To investigate the expression of GFAP in rat hypothalamus after acute heat stress. Methods The rats were caged in a experimental incubator for 60 minutes,the temperature within the incubator was adjusted to 24℃,34℃,38.5℃ or 42℃,the humidity was 60%.Single anti-GFAP immunohistochemical(ABC) method and anti-Fos and GFAP double immunohistochemical method were used to observe the expression of GFAP in hypothalamus in different ambient temperatures after heat stress. Results The GFAP-positive cells were rare in hypothalamus at 24℃,however it was increased in many nuclei(anterior hypothalamic area,paraventricular nucleus,arcuate nucleus,suprachiasmatic nucleus and supraoptic nucleus)at 34℃ and peaked when ambient temperature was 38.5℃,and then decreased.However,Fos/GFAP-IR double labelled astrocytes were observed at 42℃.Conclusion Astrocytes participate in the pathophysiological process of heat stress.

Expression of TGF-?_1 and CDK4 in Normal Esophageal Mucosa and Esophageal Squamous Cell Carcinoma / 中国医师杂志

Objective To study the roles of TGF-? 1 and CDK4 in esophageal squamous cell carcinoma (SCC).Methods The expression of TGF-? 1 and CDK4 was examined in 50 cases of esophageal squamous cell carcinoma and 10 cases of normal esophageal mucosa with SABC immunohistochemical technique. All the samples were stained by HE to confirm their pathologic characters. The number of positive cells was counted under microscope. The data were analyzed with x 2 tests and t test.Results TGF-? 1 and CDK4 positive cells were significantly higher in esophageal SCC than those in the normal esophageal mucosa (P

Inhibitory effect of Epibueropyridium A extracted from scrophularia ningpoensis on aldose reductase / 第二军医大学学报

Objective:To investigate the inhibitory effect of Epibueropyridinium A on aldose reductase.Methods: Aldose reductase was extracted from cattle crystalline lens.Besides Epibueropyridinium A,the reactive system also contained DL-glyceraldehyde,aldose reductase,and NADPH.The activity changes of aldose reductase were detected at 340 nm.Epalrestat was taken as the positive control.The inhibitory type,Ki and IC_(50) were determined by double reciprocal plot,quadratic drawing,and drawing of inhibitor's concentration to inhibitory ratio,respectively.Results: Epibueropyridinium A significantly inhibited the activity of aldose reductase in a competitive manner,with IC_(50) being 4.2 ?g/ml and Ki being 4.88 ?g/ml.Conclusion: Epibueropyridinium A is competitive inhibitor of aldose reductase.

Determination of paracetamol in the Vc Yinqiao Tablets by diffuse reflectance FTIR / 中成药

Objective: To establish a method for determination of paracetamol in Vc Yinqiao tablets (Vc, paracetamol, Flos lonicerae, Fructus Forsythies), etc. Methods: Diffuse Relectance FTIR was used to determinate paracetamol in the Vc Yinqiao tablets. Results: The content of paracetamol could be obtained directly, and the relationship between the absorbance and the concentration was linear. The average recovery of paracetamol was 98.40, and RSD was 3.18%. Conclusion: The method is simple, fast and reliable. It has been proved to be a good practice for determination of Vitamin C in the Vc Yinqiao Tablets.

PROLIFERATION AND DIFFERENTIATION OF NEURAL STEM CELLS IN THE SUBVENTRICULAR ZONE AND SUBGRANULAR ZONE IN OLD RATS AFTER FOCAL CEREBRAL ISCHEMIA / 解剖学报

Objective The proliferation and differentiation of neural stem cells in the subventricular zone(SVZ) and subgranular zone(SGZ) have been observed in normal old rats or treated by middle cerebral artery occluded(MCAO) and sham operation respectively. Methods Old rats were used to make models of middle cerebral artery occluded(MCAO).By BrdU pulse labeling and immunohistochemical single staining,the change of the number of BrdU positive cells was observed in SVZ and SGZ of normal rats or cerebral ischemia rats after MCAO 3d,7d,14d,21d,28d and sham operation respectively.By BrdU accumulating label and immunohistochemical double staining,BrdU/NeuN and BrdU/GFAP positive cells were determined in SVZ and SGZ of aged rats after MCAO 14d. Results BrdU positive cells were found in bilateral SVZ and SGZ of all groups above mentioned.Compared with normal and sham groups,BrdU positive cells of SVZ and SGZ in cerebral ischemia rats were strikingly increased.BrdU positive cells reached the peaks in SVZ at 7d and in SGZ at 14d after MCAO.At 14d after MCAO,BrdU/NeuN(0.98%) or BrdU/GFAP(12.56%) double labled cells were observed in SVZ,but no double labled cell was observed in SGZ.Conclusion The focal cerebral ischemia markedly stimulated proliferation of neural stem cells in SVZ and SGZ in old rats,and some proliferative cells in SVZ can differentiate into neurons or glial cells.